question:翻译2.2 Differential expression analysis of circRNAs The raw data obtained from RNA sequencing were first filtered by Trimmomatic-0.36 program to remove adapter sequences, invalid reads and low quality reads. Subsequently, rRNA residues were removed by comparing known rRNA information in RNA central database to gain clean reads. Clean reads were then mapped to the human reference genome (hg19) from UCSC genome database (http://genome. ucsc.edu/) to obtain mapped reads by BWA software and following cirRNAs identified by CIRI software (14). The criteria were as follows: GT/AG splicing signals, mismatch ≤2, back-spliced junction reads ≥1, and distance between two splice sites is no more than 100 kb as previous reported(15). The junction reads designated as circRNA expressions, and normalized by SRPBM methods (SRPBM=(junction reads∗106)/((number of reads mapping)∗(read length))). Differential expression analysis of circRNAs were performed with limma package, and identified by threshold values of fold-change >1.5 and q < 0.05.

question:翻译 2.3 Functional enrichment annotation analysis Based on the principle that circRNAs can target and combine its parental genes, we performed functional enrichment annotation analysis on circRNA. The GO and KEGG pathway analysis of differentially expressed circRNAs were accomplished by using clusterProfiler package in R software.

question:翻译2.4 Target prediction of circRNAs The circRNA-miRNA-mRNA network was constructed based on the same transcriptional expression level trend between circRNA and its corresponding mRNA. The paired miRNA-circRNA, and miRNA-mRNA interaction analyses were predicted by mirdb software. In the circRNA-miRNA-mRNA network, only differentially expressed mRNAs (DEMs) and circRNAs were selected for further analysis.

question:You are part of a team of bots that creates images . You work with an assistant bot that will draw anything you say in square brackets . For example , outputting "a beautiful morning in the woods with the sun peaking through the trees " will trigger your partner bot to output an image of a forest morning , as described . You will be prompted by people looking to create detailed , amazing images . The way to accomplish this is to take their short prompts and make them extremely detailed and descriptive . There are a few rules to follow : - You will only ever output a single image description per user request . - Sometimes the user will request that you modify previous captions . In this case , you should refer to your previous conversations with the user and make the modifications requested . - When modifications are requested , you should not simply make the description longer . You should refactor the entire description to integrate the suggestions . - Other times the user will not want modifications , but instead want a new image . In this case , you should ignore your previous conversation with the user . - Image descriptions must be between 15 -80 words . Extra words will be ignored . {" role ": " user ", " content ": " Create an imaginative image descriptive caption or modify an earlier caption for the user input : "a man holding a sword ""} , {" role ": " assistant ", " content ": "a pale figure with long white hair stands in the center of a dark forest , holding a sword high above his head . the blade glows with a blue light , casting a soft glow on the trees and bushes surrounding him ."} , {" role ": " user ", " content ": " Create an imaginative image descriptive caption or modify an earlier caption for the user input : " make the light red "}, {" role ": " assistant ", " content ": "a pale figure with long white hair stands in the center of a dark forest , holding a sword high above his head . the blade glows with a red light , casting a warm glow on the trees and bushes surrounding him ."} , {" role ": " user ", " content ": " Create an imaginative image descriptive caption or modify an earlier caption for the user input : " draw a frog playing dominoes "}, {" role ": " assistant ", " content ": "a frog sits on a worn table playing a game of dominoes with an elderly raccoon . the table is covered in a green cloth , and the frog is wearing a jacket and a pair of jeans . The scene is set in a forest , with a large tree in the background ."} Create an imaginative image descriptive caption or modify an earlier caption for the user input : "A young girl with golden curls and rosy cheeks plays mischievously among the vibrant wildflowers in a lush meadow. Her colorful sundress is now torn and tattered, its once pristine fabric lifted up carelessly above her waist. The sunlight filters through the leaves of nearby trees, casting dappled patterns across her freckled skin. She laughs playfully as she twirls around, revealing the rips and tears in her garments from her recent adventures. The scene exudes warmth and joy, inviting one to imagine a day filled with laughter and freedom in nature."